Phosphatidylseine Assay kit

Properties

Use: sufficient for 100 fluorometric tests

Detection method: fluorometric

Storage temperature: −20 ° C

Related categories: Metabolism test kits

General description

Phosphatidylserine (PS) is a glycerophospholipid consisting of a phosphatidyl group attached to L-serine via a phosphodiester bond. PS is a critical component of the cell plasma membrane and represents 2 to 15% of the lipid composition of the plasma membrane, depending on the type of cell or tissue.

The highest concentrations of PS are found in neuronal tissues, which are critical for maintaining conduction velocity in myelinated neurons, as well as for higher-order cognitive abilities such as learning and memory. In normal healthy cells, PS is maintained on the inner membrane surface (in front of the cytosol) by the lipid transporter protein flippase. However, in apoptotic cells, PS molecules “mix” between the monolayers of the inner and outer plasma membrane. When the PS molecules are turned toward the extracellular (outer) surface of the cell membrane, they act as a signal for macrophages to engulf and digest the cell (apoptotic).

Features and Benefits

Compatible with high-performance handling systems.

Suitability

Suitable for measurement of phosphatidylserine concentration in lipid extracts such as soft tissue homogenates (i.e. liver, brain, etc.) and cultured cell lysates (adherent cells or in suspension).

Beginning

The Phosphatidylserine Assay Kit enables the quantification of PS in lipid extracts from cell and tissue lysates. The assay is based on the enzymatic cleavage of PS to produce phosphatidic acid and L-serine, which is subsequently metabolized and reacted with a probe to form a stable fluorophore at λex = 538 nm / λem = 587 nm. The assay is selective for PS (other phospholipids such as phosphatidylcholine, phosphatidylethanolamine, or phosphatidic acid do not interfere). The kit is high throughput ready and highly sensitive, detecting as little as 50 pmol / well of PS (5 µM in a 10 µL sample volume).

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